The dual monochromator optics allow the widest range of. Page 35: Setting Up The Instrument SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. SpectraMax® Plus 384 provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Cuvette Microplate wells. The guide also covers the software features, the specifications, and the accessories of the systems. Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. The SpectraMax M2e is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. The SpectraMax iD3 reader allows you to interact with your data any way you want, from anywhere you want. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. Detailed specifications of each model reader are reported in Section 7, “Specifications”. 1 software Download page. However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. microplate reader. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. AlphaScreen assays are compatible with our SpectraMax i3x, SpectraMax i3, and SpectraMax Paradigm Multi-Mode Microplate readers with the Alphascreen Detection Cartridge options. We would like to show you a description here but the site won’t allow us. Additionally, user-installable options allow the SpectraMax i3 System to. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. . Microplate readers from Molecular Devices are designed to provide consistent performance for many years. Gemini XPS Microplate Reader (or other SpectraMax reader with fluorescence detection mode) SoftMax Pro Software; Methods Set up the instrument and software. Transcreener ® ADP. Summary of Contents for Molecular Devices SpectraMax M2. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. microplate reader. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. SpectraMax readers are equipped with PathCheck Technology, which measures the pathlength in each well of a microplate and normalizes the absorbance values to a 1-cm pathlength so that they are the same as values obtained in a 1-cm cuvette. This pathlength correction is accomplished only with the use of. View your data quickly using the large touchscreen interface, export your data for analysis in the program of. 76358-624. Examples: “Press carefully to engage,” “ Do not. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The SpectraMax® iD3 Multi Mode Microplate . The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. This section contains the following topics:滤光片型光吸收微孔板读板机（酶标仪），简单易用，性价比高，检测结果精确. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. Tecan Infinite Microplate Reader. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). Examples of results are presented below for the LMax™ II 384 dedicated, top-of-the-line microplate luminometer, the Analyst ® GT Multi-Mode, Filter-Based Instrument, and the SpectraMax ® M5 Multi-Detection, Monochromator-Based Reader with both top- and bottom-read capability. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol. Every SpectraMax® 340 PC 384 microplate reader has an optical system built around a monochromator. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. Reporter Assay. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Promega's ROS-Glo H202 assay can be combined with SpectraMax readers to explore the relationship between. Nucleic Acid and Protein Quantitation. 5014038F 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 11 Applications 12 DualPhotomultiplierTubes 12The optical system of SpectraMax Plus384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 1000 nm). The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. Keywords: spectramax mini multi-mode microplate reader, spectramax mini, spectramax mini microplate reader Created Date: 5/25/2022 9:57:03 AMFully Warranty. Examples: “Press carefully to engage,” “ Do not press down. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. The dual monochromator optics allow the. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. 0 out of 5 $ 5,999. We would like to show you a description here but the site won’t allow us. BENEFITS • No filters needed • Top- and bottom-read capability • Higher sensitivity • Validation tools • Robotics compatible SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual. 5 25 75 25 50 50 50 100 0 100 结合反应 Table 2. SpectraMax® 190 absorbance plate reader enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. More Product Information. Absorbance reading with SpectraMax® microplate readers and SoftMax® Pro Software enables optical density measurement of samples in microplate or cuvette format. Eight differentSpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. 100 readings per well gives optimal Z’ values for this assay. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). 5 25 75 25 50 50 50 100 0 100 结合反应 Table 2. The SpectraMax ® iD5 Multi-Mode Microplate Reader includes an absorbance detection mode optimal for protein quantitation assays. 1. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96-. 76358-624. Prerequisites: SoftMax Pro 7. Most papers they cultured bacteria to OD600>0. SpectraMax ABS and SpectraMax ABS Plus Microplate Spectrophotometers User Guide Temperature Regulation When you power on the instrument the incubator is off. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. Download PDF. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Example: [Drawer]. Detailed specifications of each model reader are reported in Section 7, “Specifications”. Positions H21-K22 contained plate blanks of PBS only. Examples: “Press carefully to engage,” “ Do not. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm. Users can perform spectral scans from 250 nm to 750. Materials. Learn about the features, specifications and applications of SpectraMax M2/M2e Microplate Readers, a multi-detection system with dual-mode cuvette ports and dual monochromators. The SpectraMax Multi-Mode Microplate Readers are packed in a specially designed carton. The following document is intended to demonstrate setup of this instrument. Detailed specifications of each model reader are reported in Section 7, “Specifications”. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. The SpectraMax® i3 Multi-Mode Microplate Reader Detection Platform evolves with your future research needs and offers a breadth of application possibilities. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. Music: Cylinder Six by Chris Zabriskie is licensed under a Creative Commons Attribution license (microplate reader is a device used to detect chemical, biological, or physical reactions by measuring emitted light. This application note describes experimental protocols that enable the detection of relatively low numbers of cells expressing Green Fluorescent Protein. . The University of Rouen in Normandy uses the SpectraMax iD3 and. Microplate Readers. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. Create custom fluorescence assays without having to purchase expensive filter sets. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Large, high resolution touchscreen interface allows you to easily set up methods, run experiments or view tutorial videos without the need for a dedicated computer workstation. Molecular Devices. Bookings are free, but you will need to use iLab to book. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of. Multimode Microplate Reader. 303 unable to cal dark current. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial. The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. microplates or in a cuvette at a. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Table 2 summarizes the results for the uniformity test. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. The SpectraMax iD3 reader is fully compatible with the SpectraDropTM Micro Volume Microplate, enabling quantitation of precious low volume samples. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. Download PDF. , a leading provider of high-performance life science solutions and a company of Danaher Corporation (NYSE:DHR), has launched its SpectraMax® Mini Multi-Mode Microplate. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Figure 1. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. 0 out of 5 $ 5,999. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance,. 专为科研检测平台量身打造的灵活且强大的小型微孔板读板机（酶标仪），具备终点法和动力学检测模式，标准配置6块滤光片，覆盖蛋白定量、细胞活力、农药残留、ELISA实验等. This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. Figure 1. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. SpectraMax iD3 Multi-Mode Microplate Reader Unparalleled performance on a personalized platform The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. 012 fmol 0. 100 fmol 384-well (75 µL) 0. The plate reader can measure multiple readouts, including UV. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Assays vary depending on temperature or pH and luminescence microplate reader validation DATA SHEET. Maximum signal with lowest background. Step 2: Launch SoftMax Pro Software. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. For readers without a cuvette port, simply check the box next to ‘PathCheck’. Italic and boldface type are used for emphasis. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. edu) to be added as a trained user in iLab. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence intensity. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started immediately, using the preconfigured Kinetic Turbidimetric LAL protocol in SoftMax® Pro Software, with. . An enhancer is provided in the kit where applicable to further reduce background fluorescence and to increase the. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command. The SpectraMax Multi-Mode Microplate Readers are packed in a specially designed carton. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Type:Spectrophotometer. The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. #6590-0108) Fluorescence detection using the SpectraMax iD5 reader’s monochromators for. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. The guide also covers the software features, the specifications, and the accessories of the systems. 0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9The eight-channel system consists of 8 sample beams and 8 reference beams, delivering both superior precision and speed of reading across the microplate. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. com 4. Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplate312 gain calibration failed. Compare plate readers The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Find regional support. The plate reader can measure multiple readouts, including UV and visible absorbance. Microplate reader assays requiring infrared (IR) or red-shifted. Water Constant and Cuvette Reference options are available on SpectraMax microplate readers with a cuvette port. FI assay. 380 nm/Exc. 0 out of 5 $ 5,999. . Software validation, IQ/OQ/PQ and FDA 21 CFR Part 11 compliance tools are also available. Sale. 003 fmol 0. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. Read one sample or up to 384 in0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. . Spectranet customer portalWe would like to show you a description here but the site won’t allow us. Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. 0 out of 5The SpectraMax ® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. These robust readers have been placed in labs from. Nuclear Receptor. Choose from a three- (M3), four- (M4), orIn this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. Spectranet customer portalthe SpectraMax® M Series Multi-Mode Microplate Readers, for . The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. Avoid losing high signal. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. The drawing shows a standard microplate of 15 mm height covered with a lid. The Quant-iT PicoGreen dsDNA Assay Kit, when run on a SpectraMax microplate reader with SoftMax Pro Software, is a quick, sensitive detection method for double-stranded DNA. Unlock your i3/i3x instrument after shipment. This technology allows for the automated regulation of well absorbance to a cuvette equivalent path length of 1 cm, which translates to. Table 1. The SpectraMax L Luminescence Microplate Reader with the variable-volume injectors option (10 µL to 100 µL) can measure both flash and glow luminescence. In addition, the SpectraMax® iD5 can be expanded to include bottom. microplate reader. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. The SpectraMax M2 and M2e readers can be integrated with the StakMax® Microplate Stacker from Molecular Devices in a matter of minutes. The 4 injector configuration enables flash and glow BRET assays and a 2-fold improvement in speed for traditional flash and glow assays. . A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among. The screw remains inside the instrument. Other technologies such as imaging. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase. Programmable liquid handling allows for perfectly timed, automated biochemical and cell-based assays. Tryptophan, which excites maximally around 270-280 nm and has an emission peak near 350 nm in water, dominates the emission of proteins and is the mostPhysical specifications and backup power UPS Choices for SpectraMax® and Gemini™ microplate readers. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. Future-proof any laboratory with the SpectraMax® Paradigm® Multi-Mode Microplate Reader. Our SpectraTest® ABS2, FL1, LM1, SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86 The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. The Molecular Devices Gemini XS Fluorescent Microplate Reader lets you experiment with a variety of fluorophores and can even find the best excitation and emission wavelengths for new assays. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M3. IMAP FP Settings in SoftMax ProSpectraMax M5 Multi-Mode Microplate Reader. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. Cell Cytotoxicity assay procedure. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. In . The SpectraMax® i3 is Molecular Devices latest multi-mode microplate reader which delivers several applications including; Ultra-Violet to visible absorbance detection, fluorescence intensity and multi-colour luminescence. , April 14, 2022 – Molecular Devices, LLC. When combined with the SpectraDrop Micro-Volume Microplate, the reader can quantitate up to 64 samples with as little as 2 μL sample volume in a single read. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one. ELISA Plate Readers / Microplate Readers. Compact and tunable absorbance microplate readers DATA SHEET. Other features and benefits include: Eliminate changing out filters. Our specialization servicing. In addition, the SpectraMax iD5 can be expanded to. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. SpectraMax Gemini XPS Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini XPS Reader, users don’t have to worry about having the right set of filters. The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). The SpectraMax Plus 384 reader is equipped with the innovative technology patented by Molecular Devices, the PathCheck® Sensor which measures the depth of samples in a microplate through optical path length. 1. Or scan up to 384 wells at any time to determine the best assay conditions. The SpectraMax® iD5 multi-mode microplate reader is a complete laboratory solution to increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF) and tuneable fluorescence polarisation (FP) read modes. unconventional microplate reader assays, including imaging cytometry and western blots • Protocol sharing with a strong user community • Spectral optimization wizard to maximize assay signal window (SpectraMax i3 and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most publishedi3x. The Spectramax 190 has an array of data collection modes, including kinetic, endpoint, and spectral scan modes. SpectraMax® Microplate Reader Suite of Products Knowledge Base Published Dec 16, 2020 | Updated Dec 06, 2021 Products : SpectraMax i3x reader,. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. B: Selection of Excitation Wavelength. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge. AlphaScreen. The dual monochromator optics allow. The system uses two scanning monochromators to determine the optimal excitation and emission settings. Lift up the lid over the chamber to insert or remove a cuvette. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The ABS Plus reader ranges from 190 to 1000 nm in wavelength which is equivalent to having 810 free filters. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. Introduction. Devices’ StakMax® Microplate Stacker in a matter of minutes and . An ATP standard curve spanning five decades was run using the ATPlite 1step Luminescence Assay System (PerkinElmer) on the SpectraMax iD5 reader. 5065390D 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 ComputerIntegration 8 PlateControls 9 CuvetteChamber 10SpectraMax microplate readers Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. 2. Type: Clear: Add to Cart. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. The SpectraMax iD3 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. SpectraMax ® M2 Multi-Mode Microplate Reader. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). The SpectraMax L reader is available in 1- and 2-channel (Photomultiplier Tube) configurations. Microplate Readers. 470 nm). SpectraDrop High-Throughput Screening (HTS) Kit. no. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. 1. Page 35: Setting Up The Instrument SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. Assays can be better optimized by scanning across. Page 28 Chapter 5: Read Modes and Read Types 1. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The price range of a reader starts at 2. SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. settings listed below for the SpectraMax iD5 reader can be used for any SpectraMax iD5 reader. Both readers feature an 8-channel read head for fast reads and a subset feature, the temperature independent PathCheck sensor that. The chamber contains springs that position the cuvette in the proper alignment for a read. SAN JOSE, CA — Molecular Devices, LLC. Align the two slots on the bottle holder with the two pegs on the rail. However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. Blank subtraction and standard curve fitting were done automatically by the software. Molecular Interactions. The plate was read on the SpectraMax Plus384 Absorbance Microplate Reader and SpectraMax i3x Multi-Mode Microplate Reader, using a preconfigured SpectraDrop protocol in SoftMax ® Pro Software. The SpectraDrop Microplate incorporates a specially designed adapter and a slide pair whose optical clarity allows measurements in absorbance and. A new technical approach to platelet aggregation studies. Step 2: Launch SoftMax Pro Software. , a. Minimize manual manipulation of samples and data processing, and improve laboratory efficiency. Offer Ends In: 18 Days 19: 38: 21. ATP standard curves read on SpectraMax microplate readers. A SpectraMax M2 microplate reader helps minimize operating time and save costs, allowing researchers to spend more time analyzing data and generating practical knowledge. SpectraMax L Microplate ReaderThis UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. The SPECTRAmax ® GEMINI-XS is a dual-monochromator microplate fluorometer system incorporating highly sensitive optics 4 that permit experimental determinations at low-level detection limits. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. If it looks fine, then proceed. 314 low lamp level warning. Microplate reader settings. When the incubator is on, the temperature may be controlled from 2°C above ambient to 60°C. The instruments affected are the SpectraMax ® L Microplate Reader and the StakMax® Microplate Handling System. The SpectraMax Mini Multi-Mode Microplate Reader performs your favorite applications including ELISA, DNA and protein quantitation, cell viability, gene expression with a user-friendly reader. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile instrument that can measure absorbance, fluorescence and luminescence in microplates. 300 µL of Detergent Compatible Bradford reagent was added to each well. When the incubator is on, the temperature may be controlled from 2°C above ambient to 60°C. 996 (n = 3) ; for SpectraMax Plus 384 reader, r 2 = 0. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™certified. The SpectraMax® ABS Plus Microplate Reader can run . For a higher . The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. With three modes of detection for UV/Vis absorbance, fluorescence and luminescence, the SpectraMax® Mini reader is user-upgradeable - from two to three modes, providing researchers with a versatile instrument. 230-1000 nm, Monochromator, ABS, FLR (top and bottom) and LUM, with incubation. Assays vary depending on. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence. IMAP FP Settings in SoftMax Pro SpectraMax M5 Multi-Mode Microplate Reader. Cell Analysis. The SpectraMax 340PC384 features correction for varying microplate well volumes and the elimination of standard curves based on PathCheck. High sensitivity is demonstrated with a tryptophan standard curve, and a shift in peak emission is shown using a lysozyme denaturation assay. The data sheet provides detailed information on the system's optical performance, data collection and analysis, plate stacker and robot integration, and validation options. Data exemplifying linear ranges from the three different kits are presented in Figure 2. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR-FRET. Add to Cart. Contact our support team with your questions regarding Molecular Devices products and applications. configurations offer a triple-mode cuvette port, accurate temperature control, microplate shaking andThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expandthe boundaries of your research capabilities. 0 out of 5 $ 11,999. With optimized reagents, option to operate the. Detailed specifications of each model reader are reported in Section. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. The SpectraMax® iD3 multi-mode microplate reader measures absorbance, fluorescence and luminescence and is ideal for busy multi-user, multi-application labs. Read one sample or up to 384 inThe SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. Ease of use. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). ATP standard curves read on SpectraMax microplate readers. The 2-channel configuration is available with 0, 2, or 4 injectors. SpectraMax L Microplate Reader This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. : 2 Oct 12 Page 6 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The instrument is designed to regulate the temperature of the plate chamber from 5°C above ambient to 45°C. The SpectraMax M2 and M2e are versatile and reliable. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. In addition, the SpectraMax iD5Next generation SpectraMax® absorbance microplate readers. In this product video, see how the SpectraMax M5 quickly reads samples. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. With optimized reagents, option to operate the. The SpectraMax® Mini multi-mode microplate reader offers a budget friendly solution that gives you exactly what you need when you need it. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. with SpectraMax Microplate Readers Introduction Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. Products. Highly customizable low volume. Each sample has a discrete sample beam and reference beam so that each well is measured directly. The SpectraMax® ABS Plus Microplate Reader can run . The SpectraMax® iD3 and iD5 measure absorbance, fluorescence, and luminescence. 1. 6% for the on-board optics (monochromators).